repairing effects of interleukin 11 (il-11) towards high dose methotrexate-induced rat small intestinal mucositis and its impacts on t-lymphoblastic leukemia cell line
نویسندگان
چکیده
objective(s): to investigate the efficacy of interleukin 11 (il-11) towards the high dose methotrexate (hdmtx)-concurrent rat small intestinal mucositis and its impacts on the proliferation of the human t-lymphoblastic leukemia (cem) cell line. materials and methods:95 wistar rats were randomly divided into five groups, the normal control group (a), the methotrexate (mtx) control group (b), the il-11-pre-treated high-dose group (c), the post-il-11-treatment high-dose group (d) and the post-il-11-treatment low-dose group (e). after the intraperitoneal injection of mtx in the groups b-e, the rats were sacrificed at 1, 3, 5 and 7 days. the mortality, morphological and ultrastructural changes of small intestine of each group were observed. the cells were then cultured in vitro, and the mtt method was used to investigate the effects of different concentration of il-11 on cem proliferation and also on hdmtx-induced mucositis. results: il-11 could reduce the intestinal histopathological score, increase the height of small intestinal villi, promote the proliferation of intestinal lacunar cells and reduce the mortality rate of rats. the il-11 pre-treatment group exhibited the best efficacies, demonstrating significant difference with the control group (p<0.01). in addition, the proliferation of cem was not promoted, indicating that il-11 could not inhibit hdmtx. conclusion: il-11 could reduce the severity of hdmtx-induced intestinal mucositis, and improve the survival rate of experimental rats, and could be safely used as the adjuvant treatment of hdmtx in childhood leukemia.
منابع مشابه
Repairing effects of interleukin 11 (IL-11) towards high dose methotrexate-induced rat small intestinal mucositis and its impacts on T-lymphoblastic leukemia cell line
Objective(s): To investigate the efficacy of interleukin 11 (IL-11) towards the high dose methotrexate (HDMTX)-concurrent rat small intestinal mucositis and its impacts on the proliferation of the human T-lymphoblastic leukemia (CEM) cell line. Materials and Methods:95 Wistar rats were randomly divided into five groups, the normal control group (A), the methotrexate (MTX) control group (B), th...
متن کاملRepairing effects of interleukin 11 (IL-11) towards high dose methotrexate-induced rat small intestinal mucositis and its impacts on T-lymphoblastic leukemia cell line
Objective(s): To investigate the efficacy of interleukin 11 (IL-11) towards the high dose methotrexate (HDMTX)-concurrent rat small intestinal mucositis and its impacts on the proliferation of the human T-lymphoblastic leukemia (CEM) cell line. Materials and Methods: 95 Wistar rats were randomly divided into five groups, the normal control group (A), the methotrexate (MTX) control group (B), th...
متن کاملRepairing effects of interleukin 11 (IL-11) towards high dose methotrexate-induced rat small intestinal mucositis and its impacts on T-lymphoblastic leukemia cell line
OBJECTIVES To investigate the efficacy of interleukin 11 (IL-11) towards the high dose methotrexate (HDMTX)-concurrent rat small intestinal mucositis and its impacts on the proliferation of the human T-lymphoblastic leukemia (CEM) cell line. MATERIALS AND METHODS 95 Wistar rats were randomly divided into five groups, the normal control group (A), the methotrexate (MTX) control group (B), the ...
متن کاملMedium and High-Dose Methotrexate-Induced Toxicity in Children with Acute Lymphoblastic Leukemia
Background: Methotrexate has an important role in treatment of acute leukemia. We measured methotrexate level in CSF and blood of children with acute lymphoblastic leukemia to determine complications and outcomes. Materials and Methods: One hundred and twenty patients (73 male, 47 female), with mean age at diagnosis of 6.1 years (11mo_15years) entered the study. Patients were divided into two...
متن کاملProteasome Inhibition by Carfilzomib Induced Apotosis and Autophagy in a T-cell Acute Lymphoblastic Leukemia Cell Line
T-cell acute lymphoblastic leukemia is an aggressive hematologic malignancy which is usuallyassociated with unfavorable prognosis particularly in patients with refractory/relapsed disease.Therefore, development of novel therapeutic strategies is highly required for improving theoutcome of these patients. Although there are several studies evaluating the efficacy of proteasome<...
متن کاملProteasome Inhibition by Carfilzomib Induced Apotosis and Autophagy in a T-cell Acute Lymphoblastic Leukemia Cell Line
T-cell acute lymphoblastic leukemia is an aggressive hematologic malignancy which is usuallyassociated with unfavorable prognosis particularly in patients with refractory/relapsed disease.Therefore, development of novel therapeutic strategies is highly required for improving theoutcome of these patients. Although there are several studies evaluating the efficacy of proteasome<...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of basic medical sciencesجلد ۱۹، شماره ۸، صفحات ۸۳۷-۸۴۳
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023